Jie Xiao, PhD
- Johns Hopkins School of Medicine Faculty
Languages
- English
Gender
FemaleAbout Jie Xiao
Primary Academic Title
Professor of Biophysics and Biophysical Chemistry
Background
Dr. Jie Xiao is a professor of Biophysics and Biophysical Chemistry at the Johns Hopkins School of Medicine. Her research focuses on single-molecule biophysics. Her laboratory develops novel single-molecule imaging and labeling tools in single cells to study the structures, functions, and dynamics of macromolecular assemblies. For example, her lab pioneered the use of superresolution imaging and single-molecule tracking in microbiology to study bacterial cell division and transcription. She developed single-molecule gene expression reporting systems and chromosomal DNA conformation markers to probe the dynamics of gene regulation in bacterial cells. She also devotes significant efforts to developing single-molecule imaging methods with new capacities to aid biological investigations in human cells, fluids, and tissues. Her work is at the frontier of single-molecule single-cell biophysics and has enabled new quantitative understandings of essential cellular processes.
Dr. Xiao received her undergraduate degree in Biochemistry from Nanjing University in Nanjing, China. She earned her Ph.D. from Rice University in Biochemistry and Cell Biology and completed a postdoctoral fellowship in the Department of Chemistry and Chemical Biology at Harvard University. Dr. Xiao joined the Johns Hopkins faculty in 2006.
Centers and Institutes
Contact for Research Inquiries
725 N. Wolfe Street
708A WBSB, Department of Biophysics and Biophysical Chemistry
Baltimore, MD 21205
Phone: (410) 614-0338
Research Interests
Single-molecule biophysics
Lab Website
Xiao Group - Lab Website
- The objective of the Xiao Group's research is to study the dynamics of cellular processes as they occur in real time at the single-molecule and single-cell level. The depth and breadth of our research requires an interdisciplinary approach, combining biological, biochemical and biophysical methods to address compelling biological problems quantitatively. We currently are focused on dynamics of the E. coli cell division complex assembly and the molecular mechanism in gene regulation.
Selected Publications
Bohrer C.H, Yang X, Thakur S, Weng X, Tenner B, McQuillen R, Ross B, Wooten M, Chen X, Zhang J, Roberts E, Lakadamyali M, Xiao J. A Pairwise Distance Distribution Correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM. Nat. Method, 2021, 18(6), 10.1038/s41592-021-01154-y
Fang, X., Liu, Q., Bohrer, C., Hensel Z., Han W., Wang J., Xiao J., New cell fate potentials and switching kinetics uncovered in a classic bistable switch, Nat. Commun, 2018, 2018, 9(1), 2787, PMCID: PMC6050291
Lyu Z, Yahashiri A, Yang X, McCausland JW, Kaus GM, McQuillen R, Weiss DS., Xiao J. FtsN activates septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthase in E. coli. Nat Commun., 2022, 13(1):575; PMID: 36180460; PubMed Central PMCID: PMC9525312
Yang X, McQuillen R, Lyu Z, Phillips-Mason P, De La Cruz A, McCausland JW, Liang H, DeMeester KE, Grimes CL, de Boer P, Xiao J. A two-track model for the spatiotemporal coordination of bacterial septal cell wall synthesis revealed by single-molecule imaging of FtsW. Nat. Microbio., 2021 May;6(5):584-593. PMCID: PMC8085133
Yang X., Lyu Z., Miguel A., McQuillen R., Huang K.C., Xiao J., GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell-wall synthesis, Science, 2017, 355, 744-747, PMCID: PMC5851775
Courses & Syllabi
Scientific Foundation to Medicine, Johns Hopkins School of Medicine, ME.800.619.SFM1, 9/1/19
Graduate Program Affiliations
Biochemistry, Cellular, and Molecular Biology Graduate Program
Program in Molecular Biophysics
Additional Training
Harvard University, Cambridge, MA, 2006