Landon Stuart King, MD

Professional Titles

• Executive Vice Dean

Primary Academic Title

Professor of Medicine

Background

Additional Academic Titles

Professor of Biological Chemistry

Contact for Research Inquiries

Johns Hopkins Asthma and Allergy Center
Division of Pulmonary and Critical Care Medicine
5501 Hopkins Bayview Circle
Baltimore, MD 21224

Lab Website

Landon King Lab

• The Landon King Lab studies aquaporins water-specific membrane channel proteins. We hope to understand how these proteins contribute to water homeostasis in the respiratory tract and how their expression or function may be altered in disease states.

Core Facility

Pulmonary Histology Core

Research Summary

Dr. King’s research focus early in his tenure was the biology of aquaporin water channel proteins in the respiratory tract as well as other tissues.  Subsequently, he partnered with others in the division to examine mechanisms contribution the resolution of acute lung injury.

Google Scholar

http://scholar.google.com/scholar?q=king%2C+landon&btnG=&hl=en&as_sdt=0%2C21

PubMed

http://www.ncbi.nlm.nih.gov/pubmed/?term=king+ls+AND+johns+hopkins

Selected Publications

• Original Research Publications

  1. Biaggioni I, King LS, Enayat N, Robertson D, Newman JH. 1989. Adenosine produces pulmonary vasoconstriction in sheep: Evidence for thromboxane A2- prostaglandin endoperoxide receptor activation. Circ Res. 65(6):1516-1526.

  2. Duke SS, King LS, Jones M, Newman JH, Brigham KL, Forbes J. 1989. Human recombinant interleukin-2 activated sheep lymphocytes lyse sheep pulmonary microvascular endothelial cells. J Cell Immunol. 122:188-199.

  3. King LS, Fukushima M, Banerjee M, Kang KH, Newman JH, Biaggioni I. 1991. Pulmonary vascular effects of prostaglandin D2 but not its systemic vascular or airway effects are mediated through thromboxane receptor activation. Circ Res. 68(2):352-358.

  4. Fukushima M, King LS, Kang KO, Banerjee M, Newman JH. 1990. Lung mechanics and airway reactivity in sheep during the development of oxygen toxicity. J App Physiol. 69(5):1779-1785.

  5. Kubo K, King LS, Kobayashi T, Newman JH. 1992. Differing effects of nitrogen mustard and hydorxyurea on lung O2 toxicity in adult sheep. Am. Rev. Resp.Dis. 145:13-18.

  6. Newman JH, Cochran CP, Roselli RJ, Parker RE, King LS. 1993. Pressure and flow changes in the pulmonary circulation in exercising sheep: evidence for elevated microvascular pressure. Am Rev Resp Dis. 147:921-926.

  7. King LS, Nielsen S, Agre P. 1996. Aquaporin-1 water channel protein in lung: ontogeny, steroid-induced expression, and distribution in rat. J Clin Invest. 97:2183-91.

  8. King LS, Nielsen S, Agre P. 1997. Aquaporins in complex tissues: I. Developmental patterns in respiratory tract and glandular tissue of rat. Am J Physiol Cell 273:C1541-48.

  9. Nielsen S, King LS, Christensen BM, Agre P. 1997. Aquaporins in complex tissues: II. Cellular and subcellular distribution in respiratory tract and glands of rat. Am J Physiol Cell 273:C1549-61.

  10. Moon C, King LS, Agre P. 1997. Expression of Aquaporin-1 water channel protein in mouse erythro-leukemia cells: genetic regulation of corticosteroid and chemical induction. Am J Physio. Cell 273:C1562-70.

  11. Lee MD, King LS, Nielsen S, Agre P. 1997. Genomic organization and developmental expression of aquaporin-5 in lung. Chest. 111:111S-113S.

  12. Borok Z, Lubman RL, Danto SI, Zhang X-L, Zabski SM, King LS, Agre P, Crandall ED. 1998. Keratinocyte growth factor modulates alveolar epithelial cell phenotype in vitro: expression of aquaporin-5 (AQP5). Am J Resp Cell Mol Biol. 18:554-61

  13. Hoffert J, Leitch V, Agre P, King LS. 2000. Hypertonic induction of AQP5 expression through an ERK dependent pathway. J Biol Chem. 275:9070-9077

  14. Steinfeld S, Cogan E, King LS, Agre P, Kiss R, Delporte C. 2001. Abnormal distribution of AQP5 water channel protein in salivary glands from Sjgrens syndrome patients. Lab Invest. 81:143-148

  15. Tsubota K, Hirai S, King LS, Agre P, Ishida N. 2001 Defective cellular trafficking of aquaporin-5 water channel protein in Sjgrens syndrome lacrimal glands. Lancet, 357:688-689.

  16. Leitch V, Agre P, King LS. 2001. Altered ubiquitination and stability of aquaporin-1 in hypertonic stress. Proc Natl Acad Sci USA, 98:2894-2898.

  17. King LS, Choi M, Cartron J-P, Agre P. 2001. Defective urinary concentrating ability due to a complete deficiency of aquaporin-1. N Engl J Med. 345:175-179

  18. Nejsum LN, Kwon T-H, Jensen UB, Fumagalli O, Frokiaer J, Krane CM, Menon AG, King LS, Agre P, Nielsen S. 2001. Functional requirement of aquaporin-5 in plasma membranes of sweat glands. Proc Natl Acad Sci. 99:511-516.

  19. King L, Nielsen S, Agre P, Brown R. 2002. Decreased pulmonary vascular permeability in aquaporin-1 null humans. Proc Natl Acad Sci. 99:1059-1063.

  20. Kozono D, Yasui, M, King LS, Agre P. 2002. Aquaporin water channels: atomic structure and molecular dynamics meet clinical medicine. J Clin Invest. 109:1395-1399.

  21. Gresz V, Kwon T-H, Gong H, Agre P, Steward M, King LS, Nielsen S. 2004. Immunolocalization of AQP5 in rat parotid and submandibular salivary glands after stimulation or inhibition of secretion in vivo. Am J Physiol. 287:G151-161.

  22. Herrlich A, Leitch V, King LS. 2004. Proneuregulin-1 cleavage and human epidermal growth factor receptor activation, early steps in hypertonic extracellular signal-regulated kinase activation and aquaporin expression. Proc Natl Acad Sci USA 101:15799-15804.

  23. Sidhaye V, Hoffert, J, King LS. 2005. cAMP regulation of aquaporin-5: distinct acute and chronic effects in lung epithelial cells. J Biol Chem 280(5):3590-3596.

  24. Molls RR, Savransky V, Liu M, Bevans S, Mehta T, Tuder RM, King LS, Rabb H. 2006. Keratinocyte-derived chemokine is an early biomarker of ischemic acute kidney injury. Am J Physiol Renal Physiol. 290(5):F1187-1193.

  25. Sidhaye VK, Guler AD, Schweitzer KS, DAlessio F, Caterina MJ, King LS. 2006. Transient receptor potential vailloid 4 regulates aquaporin-5 abundance under hypotonic conditions. Proc Nat Acad Sci USA 103(12):4747-4752.

  26. Phatak P, Shaldivan A, King LS, Shapiro P, Regenold WT. 2006. Lithium and inositoal: Effects on brain water homeostasis in the rat. Psychopharmacology (Berl), 186(1):41-47.

  27. Endeward V, Virkki L, King LS, Supuran CT, Boron WF, Gros G. 2006. Evidence that Aquaporin 1 is a major pathway for CO2 transport across the human erythrocyte membrane. FASEB J, 12:1974-1981.

  28. Promeneur D, Liu Y, Jorge Maciel, Agre P, L King LS, Kumar N. 2007. Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei. Proc Natl Acad Sci USA, 104(7):2211-6.

  29. Liu Y, Promeneur D, Rojek A, Kumar N, Frkir J, Nielsen S, King LS, Agre P, and Carbrey JA. 2007. Aquaporin 9 is the major pathway for glycerol uptake by mouse erythrocytes, with implications for malarial virulence. PNAS 104:12560-12564.

  30. Schweitzer K, Li E, Sidhaye V, Leitch V, Kuznetsov S, King LS. 2008. Accumulation of Aquaporin-1 during hemolysin-induced necrotic cell death. Cell Mol Biol Lett 13(2): 195-210.

  31. Sidhaye VK, Schweitzer KS, Caterina MJ, Shimoda L, King LS. 2008. Shear stress regulates aquaporin-5 and airway epithelial barrier function. Proc Natl Acad Sci USA. 105(9):3345-50, 2008.

  32. Dodd-o J, Hristopoulos, Scharfstein D, Brower RG, Hassoun PM, King LS, Becker PM, Liu M, Wang W, Hassoun HT, Rabb H. 2008. Interactive effects of mechanical ventilation and kidney health on lung function in an in vivo mouse model. Am J Physiol Lung Cell Mol Physiol 296(1):L3-L11. PMCID: PMC2636954.

  33. DAlessio F, Tsushima K, Aggarwal NR, West EE, Suffredini AF, Willett MH, Britos MF, Pipeline MR, Brower RG, Tuder RM, McDyer JF, King LS. 2009. CD4+CD25+Foxp3+ Tregs resolve experimental lung injury in mice and are present in humans with acute lung injury. J Clin Invest 119(10):2898-2913. PMID: PMC9770521

  34. Gandolfo MT, Jang HR, Bagnasco SM, Ko GJ, Agreda P, Satpute SR, Crow MT, King LS, Rabb H. 2009. Foxp3+ regulatory T cells participate in repair of ischemic acute kidney injury. Kidney Int. 2009 76(7):717-29.

  35. Aggarwal NR, DAlessio FR, Tsushima k, Files DC, Damarla M, Sidhaye VK, Fraig MF, Polotsky VY, King LS. 2010. Moderate oxygen augments lipopolysaccharide-induced lung injury in mice. Am J Physiol Lung Cell Mol Physiol 298: L371-L38. PMCID: PMC 2838673.

  36. Aggarwal NR, DAlessio FR, Tsushima K, Sidhaye VK, Cheadle C, Grigoryev DN, Kathleen C. Barnes KC, and King LS. 2009. Regulatory T cell-mediated resolution of lung injury: Identification of potential target genes via expression profiling. Physiol Genomics 2010 Apr 1;41(2):109-19. PMCID:PMC2853901

  37. Hansel NN, Sidhaye V, Rafaels NM, Gao L, Gao P, Williams R, Connet JE, Beaty TH, Mathias RA, Wise RA, King LS, Barnes KC. 2010. Aquaporin 5 Polymorphisms and Rate of Lung Function Decline in Chronic Obstructive Pulmonary Disease. PLoS ONE 5(12):e14226. PMCID PMC2997058.

  38. Sidhaye R, Chau E, Breysse PN, King LS. Septin-2 mediates airway epithelial barrier function in physiologic and pathologic conditions. Am J Resp Cell Mol Biol. 2011 45(1):120-126. PMCID: PMC3145065

  39. Finanger Hedderick EL, Simmers JL, Soleimani A, Andres-Mateos E, Marx R, Files DC, King L, Crawford TO, Corse AM, Cohn RD. 2011. Loss of sarcolemmal nNOS is a common finding in acquired and inherited neuromuscular disorders. Neurology 76(22):960-967.

  40. Calvi C, Podowski M, DAlessio F, Metzger S, Lauer T, Cheadle C, Talbot C, Jie C, McGrath-Morrow S, King L, Walston J, Neptune ER. 2011. Critical transition in immune function accompanies lung senescence. PloSONE 2011;6(6):e20712. PMCID: PMC3119663.

  41. Files DC, DAlessio FR, Johnston LF, Kesari P, Aggarwal NR, Garibaldi BT, Mock JR, Simmers JL, DeGorordo SA, Murdoch J, Willis MS, Patterson C, Tankersley CG, Messi ML, Liu C, Delbono O, Furlow JD, Bodine SC, Cohn RD, King LS, Crow MT. A critical role for muscle ring finger-1 in acute lung injury associated skeletal muscle wasting. Am J Resp Crit Care Med, 2012 Apr 15;185(8):825-34. PMCID:PMC3360571

  42. Gurkan OU, He ., Zielinski R., Rabb H, King LS, Dodd-o JM, D'Alessio FR, Aggarwal N, Pearse D, Becker PM. Interleukin-6 mediates pulmonary vascular permeability in a two-hit model of ventilator-associated lung injury. Exp Lung Res 37:575-584.

  43. Sidhaye VK, Chau E, Srivastava V, Sirimalle S, Balabhadrapatruni C, Aggarwal NR, DAlessio FR, Robinson DN, and King, LS. A novel role for aquaporin-5 in enhancing microtubule organization and stability. PLoS ONE 2012;7(6):e38717. Epub 2012 Jun 8. PMCID: PMC3371026

  44. Leggett K, Maylor J, Undem C, Lai N, Lu W, Schweitzer K, King LS, Myers AC, Sylvester JT, Sidhaye V, Shimoda LA. Hypoxia-induced migration in pulmonary arterial smooth muscle cells requires calcium-dependent upregulation of aquaporin 1. Am J Physiol Lung Cell Mol Physiol 2012 Aug 15;303(4):L343-53. PMCID: PMC3423828

  45. DAlessio FR, Tsushima K, Aggarwal NR, Files DC , Garibaldi BT, Mock JR, Eto Y, Avalos CR, Rodriguez JV, Reddy SP, Pearse DB, Sidhaye VK, Hassoun PM, Crow MT, King LS. Resolution of experimental lung injury by monocyte-derived inducible nitric oxide synthase (iNOS). J Immunol 189:2234-2245, 2012. PMCID: PMC3424351

  46. Garibaldi BT, DAlessio FR, Mock JR, Files DC, Chau E, Eto Y, Drummond MB, Aggarwal NR, Sidhaye V, King LS. Regulatory Tcells reduce acute long injury fibroproliferation by decreasing fibrocyte recruitment. Am Am J Respir Cell Mol Biol. 2012 Sep 20. [Epub ahead of print]

  47. Aggarwal NR, DAlessio FR, Eto Y, Chau E, Avalos C, Waickman AT, Garibaldi BT, Mock KJR, Files DC, Sidhaye V, Polotsky VY, Powerll J, Horton M, King LS. Macrophage adenosine receptor 2a (A2Ar) protects against oxygen-induced augmentation of experimental lung injury. Am J Resp Cell Mol Biol. 2012. (in press).

Graduate Program Affiliations

• Cellular and Molecular Medicine Graduate Program

Professional Activities

• American Heart Association, Member, 1/1/05 - 1/1/08
• American Society of Clinical Investigation, Member, 1/1/06 - 12/31/06
• American Thoracic Society, Member, 1/1/04
• American Thoracic Society, Member, 1/1/09 - 12/31/09
• American Thoracic Society Nominating Committee, Chair, 1/1/05 - 1/1/07
• American Thoracic Society Nominating Committee, Chair, 1/1/09 - 1/1/11
• Interurban Clinical Club, Member, 1/1/11 - 12/31/11
• Maryland Thoracic Society, Member, 1/1/99 - 1/1/01
• NIH/National Heart Lung and Blood Institute, Member, 1/1/05 - 12/31/05
• NIH/National Heart Lung and Blood Institute, Member, 1/1/07 - 12/31/07
• NIH/National Heart Lung and Blood Institute, Member, 1/1/08 - 12/31/09
• NIH/National Heart Lung and Blood Institute, Member, 1/1/10 - 12/31/10

Additional Training

• Residency: General Internal Medicine; Johns Hopkins University School of Medicine (1992)
• Fellowship: Pulmonary and Critical Care Medicine; Johns Hopkins Bayview Medical Center (1997)