Varsha Singh, PhD
Highlights
Languages
- English
Gender
FemaleJohns Hopkins Affiliations:
- Johns Hopkins School of Medicine Faculty
About Varsha Singh
Primary Academic Title
Assistant Professor of Medicine
Background
Dr. Singh's Lab seeks to understand mechanisms that regulate intestinal epithelial cell function during homeostasis and pathophysiological conditions. We are working to understand the trafficking and molecular regulation of membrane proteins in diarrheal diseases, epithelial stem cell differentiation during homeostasis, and chronic inflammatory conditions including IBD, and T1D. To investigate these mechanisms at the tissue, cell, and molecular levels, we apply in vivo (mouse transgenics), ex vivo (2D, 3D organotypic culture), imaging (live, confocal/2photon), and RNA sequencing technologies. The broader goal of our research is to exploit signaling pathways to suggest targeted approaches for new treatment strategies for GI diseases.
Contact for Research Inquiries
720 Rutland Avenue
Johns Hopkins School of Medicine
Baltimore, MD 21205
Research Interests
cellular physiology, Intestinal ion transport, membrane trafficking
Lab Website
Varsha Singh
- The Singh Lab does basic and translational research on intestinal ion transport, cellular physiology, and membrane trafficking in diabetic disorders.
Selected Publications
Singh V, Yang J, Cha B, Chen TE, Sarker R, Yin J, Avula LR, Tse M, Donowitz M. (2015) Sorting Nexin 27 regulates basal and stimulated Brush Border Trafficking of NHE3 (Mol Biol Cell. 2015 Jun 1;26(11):2030-43. (F1000 recommendation)
Cha B, Yang J, Singh V, Zachos NC, Sarker RI, Chen TE, Chakraborty M, Tse CM, Donowitz M.PDZ Domain Dependent Regulation of NHE3 Occurs by Both Internal Class II and C-terminal Class I PDZ Binding Motifs. J Biol Chem. 2017 Mar 10.[Epub ahead of print]
Jianbo Yang; Rafiquel Sarker; Varsha Singh; Prateeti Sarker; Jianyi Yin; Tian-e Chen; Raghothama Chaerkady; Xuhang Li; Chung Ming Tse; Mark Donowitz The NHERF2 sequence adjacent and upstream of the ERM binding domain affects NHERF2-ezrin binding and dexamethasone stimulated NHE3 activity Biochem J. 2015 Aug 15;470(1):77-90
Tiane Chen, Hetal S. Kocinsky, Rakhilya Murtazina, Boyoung Cha, Jianbo Yang, Tse CM, Varsha Singh, Rafiquel Sarker, Robert Cole, Peter S. Aronson, Hugo deJonge and Mark Donowitz. (2014) Cyclic GMP Kinase II (cGKII) Inhibits NHE3 by Altering its Trafficking and Phosphorylating NHE3 at Three Required Sites: Identification of a Multifunctional Phosphorylation Site (J Biol Chem 290(4):1952-65.)
Yang, J., Singh, V., Chen, T. E., Sarker, R., Xiong, L., Cha, B., Jin, S., Li, X., Tse, C. M., Zachos, N. C., and Donowitz, M. (2014) NHERF2/NHERF3 Protein Heterodimerization and Macrocomplex Formation Are Required for the Inhibition of NHE3 Activity by Carbachol. J Biol Chem 289, 20039-20053