Sometimes toxins, such as hazardous wastes and industrial byproducts, seep into groundwater, the source of our drinking water. One such pollutant is perchlorate, a chemical compound used in rocket fuels, fireworks, fertilizers and other materials. The compound is thought to contribute to health issues in humans such as hypothyroidism, the decreased production of hormones from the thyroid gland, which can impact development.

In a new study published May 25, 2020, in the journal Nature Structural & Molecular Biology, researchers at Johns Hopkins Medicine, Vanderbilt University and the University of California, Irvine, report on the mechanism that perchlorate uses to impact and damage normal functioning of the thyroid gland.

The findings, they say, suggest that an acceptable safe concentration of perchlorate in drinking water is one-tenth what it was previously thought to be.

The researchers focused on how perchlorate blocks a main route by which iodide, the negatively charged form of the element iodine, enters thyroid cells. Iodide helps the thyroid make hormones that are essential to the body’s regulation of metabolism, temperature and other important functions.

Thyroid cells transport iodide from the blood into the interior of the cell by using a protein called the sodium/iodide symporter, also known as NIS. Like other cellular transport systems, a “lock-and-key” approach is used to move iodide, with NIS acting as the lock and sodium as the key. Sodium fits into NIS at two binding sites to unlock the protein, enabling iodide to pass through and accumulate inside a thyroid cell.

The team, led by L Mario Amzel, Ph.D., professor of biophysics and biophysical chemistry at the Johns Hopkins University School of Medicine, and Vanderbilt University researcher Nancy Carrasco, M.D., determined that perchlorate blocks NIS by latching onto the protein and changing its shape. Less sodium binds to the misshaped protein, thereby significantly lowering the amount of iodide that can be moved into thyroid cells.

The researchers studied how varying concentrations of perchlorate affects iodide transport by first growing cells that expressed the gene SLC5A5, which encodes the instructions for building NIS. Next, perchlorate and radioactive iodide were placed outside of some of the cells and just radioactive iodide outside the others. Finally, the researchers tracked how much glowing iodide was allowed to enter the cells in both groups. They found that there was much less iodide inside thyroid cells treated with perchlorate than in untreated ones, even at very low concentrations of the chemical.

In May 2020, the U.S. Environmental Protection Agency (EPA) ruled not to place regulations on the amount of perchlorate that can be allowed in drinking water. The findings from the new study strongly suggest that this environmental pollutant is more hazardous than previously thought, raising serious concern about the decision.

“We hope that these findings will prompt the EPA to change its mind,” Amzel says.

Correction: An earlier version of this news release misidentified NIS as a channel protein. The news release has also been updated to clarify the acceptable safe concentration of perchlorate.