Thaw ES cell frozen stock and culture: 2-3 days
Passage to 10 cm dish and culture: 2-3 days
Electroporation of ES cells: 24 hours
Start Drug Selection: 8-10 days
Pick Colonies onto 96-well plates: 2-3 days
Split 1:2 onto two 96 well plates: 1 master feeder plate & 1 gelatin coated: 2-3 days
Freeze down master plates (-80) & process gelatin plates for DNA
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