Selectable Marker | Selection | Selection Agent | Working Conc. |
Neomycin resistance | positive | G418 sulfate | 180-1000 ug/ml |
Puromycin resistance | positive | Puromycin dihydrochloride | 2-6 ug/ml |
Hygromycin resistance | positive | Hygromycin B | 150-200 ug/ml |
Blasticidin resistance | positive | Blasticidin S HCL | 5 ug/ml |
Thimidine kinase | negative | Gangciclovir | 2 uM |
Thimidine kinase | negative | FIAU | 200 nM |
Diphtheria toxin | negative | none required | N/A |
Hypoxanthine (hprt) phosohoribosyltransferase | positive | HAT components: |
1X: |
Hypoxanthine (hprt) phosohoribosyltransferase | negative | 6-Thioguanine | 10uM |
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