1. First bring your cells to a single cell suspension.
2. Prepare your haemocytometer with coverslip.
3. Mix cells well and take a 10 ul sample of your cell suspension and touch the end of your pipet to the edge of the coverslip. Slowly fill chamber with capillary action. Do not overfill, too much liquid will raise coverslip resulting in an overestimation of cell number.
4. Count cells in one counting area. Be consistent on how you count cells, on/off lines etc.
5. Count four grids, average to obtain your count.
6. To calculate the number of cells/ml of medium, multiply the # of cells x 10,000.
i.e. if 100 cells are counted in 1x10E4 ml, then there are 1x10E6 cells in 1 ml.
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