Total Volume |
48 mls |
96 mls |
145 mls |
290 mls |
434 mls |
DMEM |
40 mls |
80 mls |
120 mls |
240 mls |
360 mls |
FBS |
7 mls |
14 mls |
22 mls |
44 mls |
65 mls |
NEAA |
.5 mls |
1.0 mls |
1.5 mls |
3.0 mls |
4.5 mls |
Glutamine |
.5 mls |
1.0 mls |
1.5 mls |
3.0 mls |
4.5 mls |
LIF |
5 ul |
10 ul |
15 ul |
30 ul |
45 ul |
B- mercaptoethanol |
3 ul of 1:10 dilution |
6 ul of 1:10 dilution |
1 ul |
2 ul |
3 ul |
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